The ex vivo addition of BD GolgiStop?, a protein transport inhibitor containing monensin, to in vitro- or in vivo-stimulated lymphoid cells blocks their intracellular protein transport processes. This results in the accumulation of cytokines and/or proteins in the Golgi complex. The increased accumulation of cytokines in the cell enhances the detectability of cytokine-producing cells with flow cytometric analysis.

Investigators should note that the appearance of BD GolgiStop? may range in color from clear (colorless) to a light yellow.